Correction: Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency

Correction: Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency

[This corrects the article DOI: 10.1371/journal.pone.0190192.].

Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency

We describe a method for measuring genome editing efficiency from in silico analysis of high-resolution melt curve data. The melt curve data derived from amplicons of genome-edited or unmodified target sites were processed to remove the background fluorescent signal emanating from free fluorophore and then corrected for temperature-dependent quenching of fluorescence of double-stranded DNA-boun...

Gaussian decomposition of high - resolution melt curve derivatives for measuring 1 genome - editing efficiency 2 3 4

Correction: High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

[This corrects the article DOI: 10.1371/journal.pone.0114632.].

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

With the goal to generate and characterize the phenotypes of null alleles in all genes within an organism and the recent advances in custom nucleases, genome editing limitations have moved from mutation generation to mutation detection. We previously demonstrated that High Resolution Melting (HRM) analysis is a rapid and efficient means of genotyping known zebrafish mutants. Here we establish o...